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| Registros recuperados: 11 | |
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| Yáñez,Romina; Bastías,Roberto; Higuera,Gastón; Salgado,Oscar; Katharios,Pantelis; Romero,Jaime; Espejo,Romilio; García,Katherine. |
| Background The surveillance of Vibrio parahaemolyticus in the Chilean coast has been mainly performed by multiplex PCR amplification of three different hemolysin genes, which are specie-specific virulence factors. These genes are also employed in the determination of V. parahaemolyticus pathogenic load in seafood and for characterization of pathogenic strains associated to diarrhea cases in human. During environmental surveillance that we performed every summer, we occasionally observed a thermolabile hemolysin (tlh) PCR product of a slightly smaller size than expected, which was coincident with low loads of V. parahaemolyticus in the environment. In order to understand this observation, we probed the specificity of tlh primers for the detection of V.... |
| Tipo: Journal article |
Palavras-chave: Hemolysin; Multiplex PCR; Pathogen surveillance; Virulence factor; Vibrio parahaemolyticus. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600012 |
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| Melo,Ligia Maria Rodrigues de; Almeida,Dulce; Hofer,Ernesto; Reis,Cristhiane Moura Falavina dos; Theophilo,Grace Nazareth Diogo; Santos,André Felipe das Mercês; Vieira,Regine Helena Silva dos Fernandes. |
| Ten out of fifty fresh and refrigerated samples of shrimp (Litopenaeus vannamei) collected from retailers in Natal (Rio Grande do Norte, Northeastern Brazil) tested positive for Vibrio parahaemolyticus. The Kanagawa test and multiplex PCR assays were used to detect TDH and TRH hemolysins and the tdh, trh and tlh genes, respectively. All strains were Kanagawa-negative and tlh-positive. Antibiotic susceptibility testing was done for seven antibiotics by the agar diffusion technique. Five strains (50%) presented multiple antibiotic resistance to ampicillin (90%) and amikacin (60%), while two strains (20%) displayed intermediate-level resistance to amikacin. All strains were sensitive to chloramphenicol. Intermediate-level susceptibility and/or resistance to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vibrio parahaemolyticus; Litopenaeus vannamei; Multiple antibiotic resistance. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400032 |
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| Serra, Carmen Lucia Martins; UFMA; Cavalcante, Paulo Roberto Saraiva; UFMA; Alves, Lucia Maria Coelho; UFMA; Nascimento, Adenilde Ribeiro; UFMA; Diniz, Silvia Cristina Costa de Santana de; UFMA. |
| Avaliação dos parâmetros físico-químicos e isolamento de Vibrio parahaemolyticus em águas do estuário do rio Anil (São Luís-MA, Brasil), em bancos naturais de moluscos. Foi utilizada a técnica do Número Mais Provável (NMP) com enriquecimento e subseqüente isolamento em agar tiossulfato-citrato-sacarose-sais-biliares (TCBS). Adicionalmente, foram monitorados “in situ” alguns parâmetros físico-químicos. As determinações para o pH variaram (7,25 a 7,90), O2 (0,20 a 1,2mg/L), T°C (29 a 32°C), S‰ (5 a 28,0 UPS), NH4+ (0,04 a 3,41mg/L), NO2- (0,10 a 1,91mg/L), PO43- (0,03 a 4,88mg/L). Em 56,1% na estação I e 55,4% na estação II, apresentaram positividade, para V. parahaemolyticus, com níveis entre 1,1x10 e > 1,1x105NMP/mL. Os resultados exigem uma atenção... |
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Palavras-chave: 2.01.00.00-0 Biologia Geral parâmetros físicos e químicos; Vibrio parahaemolyticus; Estuário; Nutrientes; Análises de água 2.01.00.00-0 Biologia Geral. |
| Ano: 2003 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/1978 |
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| Fouche, Dominique. |
| De nombreuses bactéries, peuplant habituellement les zones estuariennes, se révèlent être de plus en plus fréquemment des agents de maladies dont les vecteurs principaux sont les coquillages et leur consommation par l'homme. Le présent travail synthétise l'état des connaissances sur les vibrios, en particulier Vibrio cholerae, Vibrio parahaemolyticus et Vibrio vulnificus, bactéries pour lesquelles il existe une abondante littérature. L'écologie, le pouvoir pathogène et les milieux de culture les plus couramment utilisés en vue de leur détection sont présentés. Enfin, leur interaction avec les mollusques bivalves, leur survie dans ceux-ci et le problème de l'épuration conséquente de ces derniers sont abordés. |
| Tipo: Text |
Palavras-chave: Vibrio cholerae; Vibrio parahaemolyticus; Vibrio vulnificus; Bactéries indigènes; Estuaires; Bivalves; Purification. |
| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/00408/51929/52554.pdf |
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| Martinez-urtaza, Jaime; Powell, Andy; Jansa, Josep; Castro Rey, Jose Luis; Paz Montero, Oscar; Garcia Campello, Marta; Zamora Lopez, Ma Jose; Pousa, Anxela; Faraldo Valles, Ma Jose; Trinanes, Joaquin; Hervio-heath, Dominique; Keay, William; Bayley, Amanda; Hartnell, Rachel; Baker-austin, Craig. |
| We describe an outbreak of seafood-associated Vibrio parahaemolyticus in Galicia, Spain in on 18th of August 2012 affecting 100 of the 114 passengers travelling on a food banquet cruise boat. Epidemiological information from 65 people was available from follow-on interviews, of which 51 cases showed symptoms of illness. The food items identified through the questionnaires as the most probable source of the infections was shrimp. This product was unique in showing a statistically significant and the highest OR with a value of 7.59 (1.52–37.71). All the nine strains isolated from stool samples were identified as V. parahaemolyticus, seven were positive for both virulence markers tdh and trh, a single strain was positive for trh only and the remaining strain... |
| Tipo: Text |
Palavras-chave: Seafood; Tdh; Trh; Vibrio parahaemolyticus; PNW clone; ST 36. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00311/42181/41482.pdf |
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| Vieira,Regine Helena Silva dos Fernandes; Rodrigues,Dália dos Prazeres; Menezes,Everardo A.; Evangelista,Norma Suely de Santana; Reis,Eliane M.F. dos; Barreto,Leopoldo Melo; Gonçalves,Flávia Araújo. |
| The presence of faecal contamination and pathogenic microorganisms in samples of dry and wet sand collected from three major beaches in Fortaleza, Ceará State, Brazil: (Praia do Mucuripe, Praia do Futuro and Praia do Caça e Pesca), during the period of May 1999 to January 2000 was evaluated. Praia do Caça e Pesca had the highest incidence of E. coli in dry sand (56%) followed by Praia do Mucuripe (28%) and Praia do Futuro (16%). In wet sand, results were 48%, 28% and 24% for Praia do Caça e Pesca, Praia do Futuro and Praia do Mucuripe, respectively. Only two samples from Praia do Futuro, one from dry sand and another one from wet sand, were positive for Salmonella. V. parahaemolyticus was isolated from four samples from Praia do Caça e Pesca (two from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sand contamination; Yeasts; Faecal coliforms; Vibrio parahaemolyticus; Salmonella. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200001 |
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| Coutard, Francois; Pommepuy, Monique; Loaec, Solen; Hervio Heath, Dominique. |
| Aims: This work investigates the maintenance of viability and potential virulence of Vibrio parahaemolyticus in a viable but nonculturable population (VBNC) state by reverse transcription-polymerase chain reaction (RT-PCR). Methods and Results: Housekeeping genes, 16S-23S rDNA and rpoS, as well as virulence genes, tdh1 and tdh2, were selected and detected by PCR in a pathogenic strain of V. parahaemolyticus (Vp4). Their expression was then studied by RT-PCR in V. parahaemolyticus Vp4 cultivated in rich medium at 37degreesC. The 16S-23S rDNA and rpoS, tdh1, tdh2 genes were transcripted at the mid-logarithmic, stationary and late stationary phases, corresponding to various physiological states. The expression of these genes was also studied by RT-PCR in a... |
| Tipo: Text |
Palavras-chave: Vibrio parahaemolyticus; Viable but nonculturable state; Tdh2; Tdh1; RT PCR; RpoS; Environment; 16S 23S rDNA. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-1098.pdf |
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| Vieira,Regine Helena Silva dos Fernandes; Sousa,Oscarina Viana de; Costa,Renata Albuquerque; Theophilo,Grace Nazareth Diogo; Macrae,Andrew; Fonteles Filho,Antonio Adauto; Rodrigues,Dália dos Prazeres. |
| The aims of this study were to count and identify sucrose positive and negative vibrios isolated from cultivated Crassostrea rhizophorae oysters during their growing cycle. Every month for 12 months, 10 to 18 oysters were collected for study. Collections occurred at the Center for Studies of Coastal Aquaculture (CSCA), which is associated with the Institute of Marine Science, Labomar, located in Euzebio, Ceará, Brazil. Approximately 150 oysters and their intervalvular liquor were studied. Vibrio Standard Plates Counts (SPC) from oyster meat and their intervalvular liquor varied from 25 to 59,000,000 CFU/g. For most of the 12 months of the oysters' life, it was possible to identify Vibrio parahaemolyticus. Vibrio carchariae was identified in four... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vibrio spp; Oyster; Public health; Vibrio parahaemolyticus; Vibrio carchariae. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100013 |
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| Registros recuperados: 11 | |
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